| 產(chǎn)品詳情 |
| Edit |   |
|---|
| Antigenic Specificity | ELL |
|---|
| Clone | polyclonal |
|---|
| Host Species | Rabbit |
|---|
| Reactive Species | human, mouse |
|---|
| Isotype | n/a |
|---|
| Format | affinity purified |
|---|
| Size | 100 μl, 200 μl |
|---|
| Concentration | > 1 mg/ml |
|---|
| Applications | WB. RUO |
|---|
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
|---|
| Description | ELL Antibody is a Rabbit Polyclonal antibody against ELL. Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling. |
|---|
| Immunogen | Recombinant fusion protein of human ELL. |
|---|
| Other Names | ELL; C19orf17; DKFZp434I1916; ELL1; MEN; RNA polymerase II elongation factor ELL; Eleven-nineteen lysine-rich leukemia protein |
|---|
| Gene, Accession # | ELL, Gene ID: 8178, SwissProt: P55199 |
|---|
| Catalog # | abx000806 |
|---|
| Price | |
|---|
| Order / More Info | ELL Antibody from ABBEXA |
|---|
| Product Specific References | n/a |
|---|
| 產(chǎn)品資料 |
|
|