| Edit |   |
|---|
| Antigenic Specificity | MYC Epitope Tag, internal |
|---|
| Clone | polyclonal |
|---|
| Host Species | Rabbit |
|---|
| Reactive Species | n/a |
|---|
| Isotype | n/a |
|---|
| Format | affinity purified |
|---|
| Size | 100 μg |
|---|
| Concentration | 1.0 mg/ml |
|---|
| Applications | ELISA, Immunohistochemistry, Western Blot. Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. |
|---|
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
|---|
| Description | This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
|---|
| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc. |
|---|
| Other Names | Rabbit anti-MYC Epitope Tag Antibody, Rabbit anti-c-myc, Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu |
|---|
| Gene, Accession # | n/a |
|---|
| Catalog # | 600-401-381 |
|---|
| Price | |
|---|
| Order / More Info | MYC Epitope Tag, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
|---|
| Product Specific References | Sorensen EB, Conner SD. (2010) ?-secretase-dependent cleavage initiates notch signaling from the plasma membrane. Traffic. 2010 Sep;11(9):1234-45. doi: 10.1111/j.1600-0854.2010.01090.x. Epub 2010 Jun 21.Fujikawa A, Matsumoto M, Kuboyama K, Suzuki R, Noda M. (2015) Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5. PLoS One. 2015 Mar 5;10(3):e0119361. doi: 10.1371/journal.pone.0119361. eCollection 2015. |
|---|