| 產(chǎn)品詳情 |
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| Product Name | Luminometric Calcium Assay Kit |
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| Description | Screen Quest? Luminometric Calcium Assay Kit. Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine-based kit is much more sensitive than the fluorescence-based calcium assay kits (such as Fluo-4, Fluo-3, Calcium-3 and Calcium-4). This kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring of intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues. |
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| Size | 100 plates |
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| Concentration | n/a |
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| Applications | Biossay |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 36306 |
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| Order / More Info | Luminometric Calcium Assay Kit from AAT BIOQUEST, INC. |
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| Product Specific References | n/a |
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