| 產品詳情 |
| Edit |   |
|---|
| Product Name | MutaPLATE? Lactase (TM) TaqMan (32) |
|---|
| Description | The Eagle Biosciences MutaPLATE? Laktase (TAQ-Man) real time PCR Kit contains specific primers and additional material for the detection of the T/C (-13910) polymorphism in the regulatory region of the lactase phlorizin hydrolase gene. The variable area of the regulatory region from lactase gene is amplified by PCR using genomic DNA-template. The specific primers used in the kit flank the variable area of lactase gene (LCT) and generate an amplificate of 300 bp using Taq-Man technology. The standard PCR contains additionally two sequence specific oligonucleotides marked with fluorescence dye (TaqMan probes). Both probes bind at the amplificated target-DNA which includes the single nucleotide polymorphism (SNP). Due to this, a fluorescence signal is generated and detected by the optical unit of the used real time PCR instrument. The TaqMan probe for the C-allele (mutation) is marked with FAM (510nm, green) and the TaqMan probe for the T-allele (wildtype) is marked with YAK (555 nm, yellow). Sample Types: DNA (e.g.whole blood, cheek swab). ? The Eagle Biosciences MutaPLATE? Lactase (TAQ-Man) real time PCR kit is a molecular biological test for analysis of the -13910 T/C polymorphism in the regulatory region of lactase phlorizin hydrolase gene (LPH) in open real time PCR systems (e. g. RotorGene, SmartCycler, Light Cycler, ABI, Stratagene, Amplifa). The clinical relevant C/C - genotype causes primary lactase deficiency often leading to (genetic) lactose intolerance. The Eagle Biosciences MutaPLATE? Lactase (TAQ-Man) is for research use only and is not intended for diagnostic or therapeutic procedures. |
|---|
| Size | 32 tests |
|---|
| Concentration | n/a |
|---|
| Applications | RUO |
|---|
| Other Names | MutaPLATE? Lactase (TM) TaqMan (32) |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | KF1907232 |
|---|
| Price | |
|---|
| Order / More Info | MutaPLATE® Lactase (TM) TaqMan (32) from EAGLE BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 產品資料 |
|
|